RPA. The versatile PCR replacement.
Recombinase Polymerase Amplification (RPA), is transforming our ability to amplify and detect nucleic acids in laboratory and resource-limited field settings.
Field
Point-of-care
Other settings with
minimal resources
Easily transportable, user friendly and highly sensitive, RPA is as specific as PCR amplification but is much, much faster. Results are typically generated within 3-10 minutes. And unlike PCR, the RPA reaction doesn’t require thermal or chemical melting, so there’s no need for an expensive thermoycler or any additional equipment or reagents.
Available Products
These products are for research and development purposes only as per our terms and conditions of supply. Select one of the TwistAmp® products areas below for more information or to purchase.
TwistAmp® lyophilised (freeze-dried) kits are conveniently provided in a format ideally suited to taking out in the field, point-of-care and other settings with minimal resources. TwistAmp® Liquid aims to supply bulk liquid forms of core RPA components which end-users may combine at various scales to create master mixes for their own applications.
TwistAmp® exo
Real-time fluorescent DNA amplification made possible with a TwistAmp® exo kit. Recommended for users who want to combine TwistDx’s RPA amplification technology with the use of TwistDx’s proprietary fluorescent TwistAmp® exo probe in a homogenous format. The user need only supply primers, probe, and template.
TwistAmp® Basic
DNA amplification made possible with a TwistAmp® Basic kit. Contains all the enzymes and reagents necessary for the amplification of DNA, the user needs only supply primers and template. TwistAmp® Basic has been used for solid-phase, tailed primers, aptamers, electrochemistry and microarray applications.
TwistAmp® Liquid Basic
DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template.
Perfect for:
- End-point gel electrophoresis DNA detection
- Down-stream applications (e.g. sub-cloning)
- Facilitating use of different RPA reaction volumes, or variation of component ratios.
TwistAmp® Liquid exo
Real-time fluorescent DNA amplification in a flexible liquid format. Recommended for users who want to combine RPA with the use of TwistDx’s proprietary fluorescent TwistAmp® exo Probes. In addition to the basic components, a powerful nuclease (Exonuclease III) is provided which will process TwistAmp® exo Probes during the amplification reaction itself and generate a real-time readout. The user need only supply primers, probe, dNTPs and template.
TwistAmp® exo positive control template
A ready made assay – exo positive control DNA template (for use with product TwistAmp® exo positive control oligo mix CONTROL01EXO). Useful for application development or further positive control testing. Use in combination with the TwistAmp® exo kits (lyophilised or liquid) or the TwistAmp® nfo kit, for fluorescence detection.
Supplied as a single tube containing 100µl (1000 copies per µl) DNA template.
TwistAmp® exo positive control template (one tube) product code: CONTROL01DNAE
(Use in combination with TwistAmp® exo positive control oligo mix product code: CONTROL01EXO)
TwistAmp® exo positive control oligo mix
A ready-made assay – primers plus a FAM labelled exo probe (to be used with TwistAmp® exo positive control DNA template CONTROL01DNAE). Useful for application development or further positive control testing. Use in combination with the TwistAmp® exo kits (lyophilised or liquid) or the TwistAmp® nfo kit, for fluorescence detection.
One tube provides enough oligonucleotide mix to conduct eight 50 µl RPA reactions.
TwistAmp® exo positive control oligo mix (one tube) product code: CONTROL01EXO
(Use in combination with TwistAmp® exo positive control template product code: CONTROL01DNAE)
U-Star Disposable Nucleic Acid Lateral Flow Detection Units (pack of 20)
Units contain a lateral flow strip designed to detect a biotin and FITC (or FAM) labelled amplicon. Detection is performed in a sealed cartridge preventing cross-contamination from amplified products. U-star disposable units can be used to detect amplification products generated when using a TwistAmp® nfo kit in combination with a TwistAmp® nfo probe and labelled amplification primer. See U-Star guide [pdf link to be provided soon] for further information.
Product code: USTAR01
Milenia HybriDetect 1
(pack of 100)
Lateral flow strips designed to detect a biotin and FITC/FAM labelled amplicon. Milenia Genline HybriDetect 1 (PDF 276 KB) lateral flow strips can be used to detect amplification products generated when using a TwistAmp® nfo kit in combination with a TwistAmp® nfo probe and labelled amplification primer.
Lateral flow strips can be purchased directly from the manufacturer below.
PCRD Nucleic Acid Detection (pack of 50)
Lateral flow strips designed to simultaneously detect two amplicons labelled with DIG/Biotin and/or FITC (or FAM)/Biotin, with the addition of a control line. PCRD is a Nucleic acid lateral flow immunoassay (NALFIA), suitable for use to detect amplification products generated when using a TwistAmp® nfo kit in combination with a TwistAmp® nfo probe and labelled amplification primer.
PCRD lateral flow strips can be purchased directly from the manufacturer below.
